Two-photon excitation STED microscopyArticle on obtaining sub-diffraction resolution in two-photon excitation (TPE) fluorescence microscopy by merging this technique with stimulated-emission depletion (STED).
Light from the darkShort article on the viewing of Non-fluorescent, light-absorbing molecules using a method that turns them into mini-lasers. Stefan W. Hell and Eva Rittweger, Nature 461, 1069-1070.
Single-molecule biology and Bioimaging Zhuang Research LabZhuang Lab at Harvard University is developing real-time fluorescence imaging methods to track the behavior of single virus particles and of single viral genomes in live cells.
Fluorescence microscopy with super-resolved optical sectionsAlexander Egner1 and Stefan W. Hell, TRENDS in Cell Biology Vol.15 No.4 April 2005
Authors: Alexander Egner1 and Stefan W. Hell, TRENDS in Cell Biology Vol.15 No.4 April 2005
Blinded by the LightPublished in 2004, the article reviews advances in light microscopy including 4Pi, STED, confocal imaging, and total internal reflection fluorescence.
Authors: Bio-IT World Magazine , Rabiya S. Tuma
Super-Resolution Microscopy Captures Molecules in MotionA new twist on a sophisticated light microscopy technique is enabling researchers to capture short videos of fast-moving cellular processes while delivering super high resolution images of whole cells.
Nonlinear structured-illumination microscopy: Wide-field fluorescence with theoretically unlimited resolutionArticle by Mats Gustafsson on saturated structured-illumination microscopy that has a 2D point resolution of less than 50 nm.
Authors: Mats G. L. Gustafsson
What is Stochastic Optical Reconstruction Microscopy (STORM)Brief introduction to Stochastic Optical Reconstruction Microscopy (STORM). Includes a reference to peer reviewed articles.
Breaking the Diffraction Barrier:Online peer reviewed article that is an overview of the two classes of super-resolution fluorescence microscopes by leading research scientist. Patterned Illumination approach includes stimulated emission depletion (STED) microscopy, RESOLFT technology (Hofmann et al., 2005), and saturated structured illumination microscopy (SSIM). Single molecule imaging that activates individual moelcules at different times includes stochastic optical reconstruction microscopy (STORM), photoactivated localization microscopy (PALM), and fluorescence photoactivation localization microscopy (FPALM)
From: , University of California San Francisco
University of California San Francisco
Authors: Bo Huang, Hazen Babcock, Xiaowei Zhuang
Citation: Cell, Volume 143, Issue 7, 1047-1058, 17 December 2010
Web Link Contact Source
Super resolution microscopyWell written up to date (as of 2012) article that overviews the types of super resolution microscopes.
iPALM: resolving the third dimension
Three-dimensional live microscopy beyond the diffraction limitThis 2013 review techniques that are available for three-dimensional, super-resolution live microscopy are discussed. Live cell imaging requires imaging at speeds fast enough to capture dynamic processes without damaging the specimen. Techniques included are Parallelized 4Pi microscopy, 3D structured illumination microscopy, Localization based microscopy, and Reversible saturable optical fluorescence transitions microscopy (RESOLFT).
Authors: Reto Fiolka
Citation: J. Opt. 15 094002, doi:10.1088/2040-8978/15/9/094002
Shattering the diffraction limit of light: A revolution in fluorescence microscopy?
Authors: Weiss, S. (2000). Proc. Natl. Acad. Sci. USA 97: 8747-8749.
STED Microscopy: A New Chapter in Light ImagingEasy to read article on the invention of the of the STED - stimulation depleted microscopy by Stefan Hell and new directions for the instruments as the basic patent is set to expire in 2015.
rsEGFP2 enables fast RESOLFT nanoscopy of living cellsRESOLOFT uses lower intensities light than STED that is pulsed in a doughnut so that 100,000 doughnuts can be viewed at one time. This produces a much larger field of view than is available with STED.
New Innovations for Biological Imaging and Instrumentation at HHMIThe Applied Physics and Instrumentation Group (APIG) at Janelia Farms seeks to understand bioimaging challenges and to harness new technologies for advanced imaging. Three initial projects will characterize this effort: higher resolution novel microscopy, high-throughput microscopy, and correlative microscopy.
The Nobel Prize in Chemistry 2014"For the development of super-resolved fluorescence microscopy" the Nobel Prize in Chemistry 2014 was awarded jointly to Eric Betzig, Stefan W. Hell and William E. Moerner. The site includes biographic information on the lauretes, advanced background information on super resolution microscopy andpopluar information on how an optical microscope became a nanoscope. Acceptance speeches will also be included on the site.
Citation: The Nobel Prize in Chemistry 2014". Nobelprize.org. Nobel Media AB 2014. Web.
Lectures and Videos
Intracellular Fluorescent Imaging: Jennifer Lippincott-SchwartzThree part series on fluorescent imaging and the recent advances in protein markers and instrumentation that allow the visualization and tracking of events with cells at a molecular level. Part 1 is an overview of the principles of fluorescence and the use of GFP has a label for intercellular compartment. Lippincott-Schwartz shows how these labels have been used understand processes in the secretary membrane system. In part two the use of photoactivation and photobleaching (FRAP) to switch on or off specific subsets of fluorescent molecules is described. The three part describes Photo Activated Localization Microscopy (PALM) developed by Eric Betzig and Harald Hess.
Xiaowei Zhuang Lecture: Super-Resolution MicroscopyA three part iBioEducation lecture series on fluorescence imaging below the diffraction limit of light. Part one is an introduction to Super-Resolution Fluorescence Microscopy with emphases on Stochastic optical reconstruction microscopy (STORM) developed by Zhuang's laboratory. In part two the application of STORM to the study of chormosomes organization of E. coli and to the determination of the molecular structure of a synapse are presented.
Stefan Hell on nanometer-scale microscopy in biophotonicsStefan Hell discusses Stimulated Emission Depletion (STED) microscopy and other far-field optical techniques to noninvasively examine living cells at resolutions better than 20 nm.
Super Resolution Microscopy Leica Science LabThe Leica site is includes articles by staff, freelance writers, and users, tutorials, and abstracts that are linked to publications. Webinars are available on demand and require registration. The Super Resolution Microscopy contains many resourses on STED, which is part of the Leica product line.
Introductions and Briefs
Advances in high-resolution imaging – techniques for three-dimensional imaging of cellular structuresThis 2012 commentary provides an excellent overview of microscopic techniques used in the 3D imaging of cellular structures. Advances in electron microscopy tomography and fluorescence super-resolution techniques including STED, Single-molecule-localization-based super-resolution, Plane illumination microscopy, and Structured illumination microscopy are reviewed. There is an insightful comparison of super resolution techniques to confocal microscopy in terms of difficulty of preparation, processing and live cell.
From: Super-Resolution Technology Core, University of New Mexico
Super-Resolution Technology Core, University of New Mexico
Authors: Diane S. Lidke and Keith A. Lidke
Citation: June 1, 2012 J Cell Sci 125, 2571-2580., doi: 10.1242/jcs.090027
Web Link Contact Source