Total Internal Reflection Fluorescence Microscopy: Introduction and Applications
Technical Knowledge for Filters and Fluorescence MicroscopyKnowledge base with an emphasis on selecting filters for fluorescence microscopy applications including TIRF, FRET, Ca2+ ratiometry, Raman, Confocal, Qdots, flow cytometry and all types of fluorescence miccroscopes. Chroma filters are widely used in microscopy.
Green Fluorescent ProteinWhat is Green Fluorescent Protein and why is it so important.
A blog on fluorescent microscopy in biological researchgreenfluorescentblog contains information on fluorescent microscopy techniques as well as current research using advanced fluorescence. This information is up to date and well organized . It is written by Gal Haimovich, a Postdoc at a leading laboratory.
Authors: Gal Haimovich
Concepts in Fluorescence Microscopy
Fluorescent ProteinsExcellent overview of fluorescent proteins used by biologists as markers of gene expression and protein targeting in intact cells and organisms in order to monitor cellular processes in living systems with fluorescence microscopy.
Nobel Prize 2008 for Green Fluorescent ProteinOsamu Shimomura, Martin Chalfie and Roger Y. Tsien jointly won the Nobel Prize in Chemistry in 2008 for discovery and development of the green fluorescent protein, GFP. Included are the biographies, lectures, interviews, photos, and documentaries on the award.
Introductions and Briefs
Fluorescence Microscope BasicsA detailed interactive animation that illustrate the basics of imaging in the Fluorescence Microscope.
A New Approach to Fluorescence MicroscopyGE & Science Prize for Young Life Scientists winning essay by Mark Bates describing his discovery of a new type of “optically switchable” fluorescent molecule, and how these molecules were used for high-resolution biological imaging.
Authors: Mark Bates
Multicolor FRET: Adding Colors to the RainbowExcellent short article discussing recent advances in FRET including the new fluorescent proteins with distinct colors that can be detected allowing six interactions to me measured at once.
Authors: Sarah C.P. Williams