Tutorials in transmission electron microscopyFor biological and material scientists who are new to TEM, but need to learn to use the technique. Includes alignment instructions.
Course Notes from Dr. Timothy S. BakerCurrent course notes for electron microscopy in pdf and PowerPoint. The Bottom Line is especially helpful and focuses basic concepts that are important for understanding the fundamental principles of transmission electron microscopy, biological specimen preparation, and three-dimensional image processing and reconstruction. Winter 2011 course is 3D Electron Microscopy of Macromolecules.
Transmission Electron Microscopy (TEM)This is a set of resources designed to accompany an introductory course on transmission electron microscopy. The level is appropriate for students with an understanding of some elementary physics.
Learn to do TEMLearn to use a transmission electron microscope...fast. This site has put together by John Rodenburg, who has been teaching graduate students to use TEMs for over twenty years (at the University of Cambridge, Sheffield Hallam and Sheffield Universities). Now includes a new section on TEM/STEM alignment.
Bioscience Electron Microscopy CourseHighlight of the site is the thirty plus lecture power point presentations developed by John Shields. Topics include specimen preparation for scanning electron and transmission electron microscopy, SEM and TEM instrument design, electron microscopy techniques, and overviews of other types of microscopy. A pdf of the Lab Manual on the preparation of biological specimens for transmission and scanning electron microscopy is available.
Development of 200kV Atomic Resolution Analytical Microscope
Focused Ion Beam Systems and Dualbeam SystemsA section on focussed ion beam instruments is included in the FEI booklet "An Introduction to Electron Microscopy". The booklet is an excellent overview of of electron microscopy and nanotechnology for students and teachers. It is available as a pdf for download.
Topics in recent studies with high-voltage electron microscopesHigh-voltage electron microscopy possesses a number of advantages that cannot be afforded by conventional electron microscopy, thus providing a unique microscopy technique in both materials science and biological science.
Authors: Hirotaro Mori
Citation: J Electron Microsc (Tokyo) (2011) 60 (suppl 1): S189-S197. doi: 10.1093/jmicro/dfr050
Optimizing Your STEM MicroscopeList of publications on optimizing the environment and optimum convergence angles for maximum performance in STEM. All publications are available on the site.
Investigations & Applications
Disease Carriers: Explore the Microscopic World of Health ScienceTransmission electron microscope images of viruses, bacteria, fungi and protozoa disease carriers.
Intracellular Aspects of the Process of Protein SecretionGeorge E. Palade – Nobel Lecture, December 12, 1974
Shades of gray matterSummary of research by Xiaokun Sun with Roger Tsien and Mark Ellisman on developing an electron opaque stain that can be genetically tagged to genes of interest and fluoresces. This would allow correlation of the sample from the electron microscope to the light microscope.
Introductions and Briefs
The Electron Microscope as an Illustration of the Wave Nature of the ElectronThe transmission electron microscope uses principles that originated in light microscopy and which therefore rely on the properties of waves. These principles are illustrated.
Electron Energy Loss SpectroscopyBrief introduction to the Principle and application of Imaging electron energy loss spectroscopy
Dynamic Transmission Electron MicroscopeIntroduction to the Dynamic Transmission Electron Microscope (DTEM) at Lawrence Livermore Laboratories that provides the ability to image transient behaviors such as how a chemical reaction, structural deformation or phase transformation takes place with an unprecedented combination of spatial and temporal resolution: nanometers and nanoseconds.
Fluctuation MicroscopyFluctuation microscopy is based on the statistical analysis of the scattering from nanoscopic volumes of the sample. It can be done using dark-field imaging in a conventional TEM. It has been used to study disordered materials including amorphous Ge and silicon and metallic glasses. This paper is a general introduction to the technique.
Electron Microscopy for Dummies (for Single Particle Reconstruction)An introduction to the single particle reconstruction electron microscopy and it's applications to biology. The site includes a brief introduction to the transmission electron microscope, and more detailed information on preparation of biological sections using grids, staining and freezing and Single Particle Reconstruction. Last update was Nov 2012.
Authors: Hanspeter Niederstrasser
Movie Mode Dynamic Transmission Electron Microscopy (DTEM)The Dynamic Transmission Electron Microscope (DTEM) at Lawrence Livermore National Laboratory has the capability of capturing micro- and nano-structural events in multiframe movies. Applications include direct viewing at the microscale of material structural changes and chemical reactions.
From: Dynamic Transmission Electron Microscope, Lawrence Livermore National Laboratory
Dynamic Transmission Electron Microscope, Lawrence Livermore National Laboratory
Authors: Rosae Hansen
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