Investigations & Applications
3D Electron Microscopy Research ProjectsSummaries of in-house research projects using electron tomography and cryo electron microscopy to study the invaginations in the mitochondrial inner membrane, functional states of the ryanodine receptor (RyR), the interaction between the ribosome and its ligands and kinetochore modulation of microtubule dynamics. An extensive list of publications is included in the site.
Three-dimensional electron crystallography of protein microcrystalsUsing a new protocal electron diffraction data from protein crystals was collected at 2.9 Å resolution using electron diffraction in a cryo electron microscope and result in. The protocol used an electron diffraction collected at resolutions of 1.7 Å. A full tilt series of 90 diffraction patterns was collected from three crystals. The technique is named ‘MicroED’.
3D-EM Web SiteThe site includes information on the 3D EM mailing list, laboraotries and events.
Introductions and Briefs
Cryo-electron tomography and Single-particle cryo-EMThe techniques development section contains summaries of the contributions of scientist at the Wadworth Center to the development of three-dimensional reconstruction of single particles and cryo-electron tomography techniques. A list of references is included.
Advances in high-resolution imaging – techniques for three-dimensional imaging of cellular structuresThis 2012 commentary provides an excellent overview of microscopic techniques used in the 3D imaging of cellular structures. Advances in electron microscopy tomography and fluorescence super-resolution techniques including STED, Single-molecule-localization-based super-resolution, Plane illumination microscopy, and Structured illumination microscopy are reviewed. There is an insightful comparison of super resolution techniques to confocal microscopy in terms of difficulty of preparation, processing and live cell.
From: Super-Resolution Technology Core, University of New Mexico
Super-Resolution Technology Core, University of New Mexico
Authors: Diane S. Lidke and Keith A. Lidke
Citation: June 1, 2012 J Cell Sci 125, 2571-2580., doi: 10.1242/jcs.090027
Web Link Contact Source
Imaging the building blocks of virusesNew microscopy techniques are illuminating viral structures and protein interaction. Surface plasmon resonance based on the optical excitation of surface plasmons is being used to detect high-resolution, label-free imaging of single viruses. Cryo electron microscopy, pushed to a resolution of 4Å can image amino acid side chains in proteins. X-ray techniques now are now able to to provide data on virus that are pieced together to form a image of the structure.
Authors: Suzanne Winter
Electron CryotomographyPeer reviewed article summarizing using cryoelectron microscopy to reconstruct 3-D structures of macromolcules, virus and small whole cells. Includes are details on the insstrumentation, preparation, limitations, and data collection.
Direct detection pays off for electron cryo-microscopyThis online journal article described improvements in image-processing techniques and electron detectors that decrease the number of images needed for single particle cryo-electron microscopy images.
A national facility for biological cryo-electron microscopyAn excellent introduction to the techniques of cryo-electron microscopy is included in this paper on the electron Bio-Imaging Centre at Diamond Light Source. Topics reviewed are single particle analysis, electron cryo-tomography, correlative microscopy, and electron crystallography.
From: electron Bio-Imaging Centre eBIC, Diamond Light Source
electron Bio-Imaging Centre eBIC, Diamond Light Source
Authors: Helen R. Saibil,a* Kay Grünewaldb and David I. Stuartb,c
Citation: Biological Crystallography, Volume 71, January 2015, 127-135 doi:10.1107/S1399004714025280
Web Link Contact Source
Software Tools For Molecular MicroscopyThe Wiki Open Content document is a comprehensive list of the software developed for cryo-electron microscopy and molecular microscopy. Software is loosely categorized by functionality. Website, contact, description, cost, and references are included for each tool. The page is well supported by the scientific community.
Authors: Voss NR, Potter CS, Smith R, Carragher B.